Item – Thèses Canada

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Numéro d'OCLC
1006756467
Lien(s) vers le texte intégral
Exemplaire de BAC
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Auteur
Khan, Vania Rhea,1974-
Titre
The effect of hyperthermia on the induction of cell death and hsp70 in neural and non-neural tissues of the rat.
Diplôme
M. Sc. -- University of Toronto, 2001
Éditeur
Ottawa : National Library of Canada = Bibliothèque nationale du Canada, [2002]
Description
2 microfiches
Notes
Includes bibliographical references.
Résumé
This thesis examines the effect of hyperthermia on the induction of cell death via apoptosis, assayed by TUNEL and active caspase 3 cytochemistry, in the adult rat brain, testis and thymus. A fever-like increase in temperature triggered apoptosis in dividing cell populations of the testis and thymus, but not in mature, postmitotic cells of the adult cerebellum. Western blot and immunocytochemical analysis of these tissues demonstrated that the induction patterns of hsp70 did not correlate with the induction of apoptosis in all three tissues. Hyperthermia-induced cell death was also examined in proliferative regions of the developing brain. The embryonic neuroepithelium and the external granule layer of the postnatal cerebellum, were susceptible to heat-induced apoptosis. The heat-sensitivity of the developing brain suggests that dividing neural cells are more prone to hyperthermia-induced cell death than mature, post-mitotic neural cells, which need to be protected because they are irreplaceable.
ISBN
0612632490
9780612632493