Item – Theses Canada

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OCLC number
946568415
Link(s) to full text
LAC copy
LAC copy
Author
Lord, Christina Ella Nickerson,
Title
Developmentally regulated and environmentally induced programmed cell death (PCD) in the lace plant (Aponogeton madagascariensis)
Degree
Ph. D. -- Dalhousie University, 2013
Publisher
Halifax, NS : Dalhousie University, 2013.
©2013
Description
1 online resource (xx, 280 leaves) :illustrations (chiefly colour)
Notes
Title from PDF title page (viewed Apr. 12, 2016).
Thesis supervisor: Dr. Arunika Gunawardena.
Includes bibliographical references (leaves 251-267).
Abstract
ABSTRACT: Programmed cell death (PCD) is pervasive in eukaryotes, playing a fundamental role in development. PCD in animals has been studied in detail, partly due to Caenorhabditis elegans, a worm whose anatomy allowed for the investigation of exactly 131 cells that die via PCD. Elucidating this complex pathway in this simple worm laid the foundation for further insights into mammalian PCD. Overall, less is known regarding PCD in plants, where cell death is broadly separated into developmentally regulated and environmentally induced. The lace plant (Aponogeton madagascariensis) undergoes developmentally regulated PCD to form perforations between longitudinal and transverse veins over its leaf surface. The optimization of protoplast isolation and induced cell death via heat shock (HS) in the lace plant is detailed here. Following HS, protoplasts displayed characteristics of PCD including: Terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling (TUNEL) positive nuclei, increases in vesicles as well as Brownian motion, and plasma membrane blebbing. Additionally, mitochondrial dynamics were investigated, and a role for the mitochondrial permeability transition pore (MPTP) was indirectly established via cyclosporine A (CsA) experimentation. The main focus of this dissertation was to elucidate cellular dynamics during developmentally regulated PCD in the lace plant, which is visibly discernable during the window stage of leaf development. A single areole within a window stage leaf was further divided into three areas based on the progression of PCD; non-PCD (NPCD) cells, early PCD (EPCD) cells, and late PCD (LPCD) cells.
Other link(s)
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Subject
Lace Plant, Programmed Cell Death, Mitochondria, Protoplasts