Item – Theses Canada

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OCLC number
890511473
Link(s) to full text
LAC copy
LAC copy
Author
Jeganathan, Senthure,
Title
Leucine-induced impairment of insulin sensitivity in skeletal muscle of healthy rats is reversible.
Degree
M. Sc. -- York University, 2011
Publisher
Ottawa : Library and Archives Canada = Bibliothèque et Archives Canada, [2012]
Description
2 microfiches
Notes
Includes bibliographical references.
Abstract
<?Pub Inc> High protein diets promote weight loss and the mTORC1/S6K1 pathway is vital to this process as it senses nutrients and regulates muscle mass. However, excess amino acids (AA) regulate a negative feedback loop causing insulin resistance. S6K1, a substrate of mTORC1, has been implicated in this, as mice lacking S6K1 have improved insulin sensitivity. I hypothesize that AA induced insulin resistance in skeletal muscle is reversible, but possibly not under pathological conditions. L6 myotubes were starved for 4 h and re-incubated with leucine (800 [mu]M) and/or insulin (100 nM). An increase in S6K1 (T389) and IRS1 (serine) phosphorylation and suppression of insulin-stimulated glucose uptake was seen in myotubes treated with leucine and insulin (P<0.01). However, these effects were reversible. This pattern of S6K1 and IRS1 phosphorylation and glucose metabolism was recaptured in skeletal muscle of healthy rats gavaged with leucine, but not in insulin resistant rats following leucine gavage.
ISBN
9780494805039
049480503X