Item – Theses Canada

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OCLC number
777303287
Link(s) to full text
LAC copy
LAC copy
Author
Wiens, Richard,1980-
Title
Evaluation of the effects of L-2-oxothiazolidine-4-carboxylate or quercetin on developing scar tissue in rats using synchrotron infrared microspectroscopy.
Degree
Ph. D. -- University of Manitoba, 2010
Publisher
Ottawa : Library and Archives Canada = Bibliothèque et Archives Canada, [2011]
Description
3 microfiches
Notes
Includes bibliographical references.
Abstract
<?Pub Inc> Synchrotron infrared microspectroscopy was used as part of a multi-disciplinary research project to evaluate the effects of two therapeutic agents, L2-oxothiazolidine-4-carboxylate (OTC) and quercetin, on the wound healing of laminectomized rats. Synchrotron light sources were used to achieve the necessary spatial resolution (~10 microns) to image the heterogeneous wound area. Our hypothesis is that post-surgical inflammation and oxidative stress following laminectomy can be reduced by systemic administration of L-2-oxothiazolidine-4-carboxylate (OTC) or quercetin, and that reducing the levels of inflammation and oxidative stress will result in better wound healing. As part of a Natural Sciences and Engineering Research Council Collaborative Health Research Project (NSERC-CHRP) to test the effects of OTC and quercetin on wound healing, male wistar rats underwent lumbar laminectomies. The rats were placed in one of three treatment groups (saline control, OTC, or quercetin), and sacrificed at three different time points (3, 21, and 63 days post surgery) in the wound healing process. Infrared data was collected on the scar tissue, and analyzed for levels of collagen, phosphates, sugars, and lipids. The 3 day animals showed little collagen, but large amounts of phosphates. The 21 day animals had larger amounts of collagen than the 3 day animals, and a decrease in phosphates. The 63 day animals had a contracted wound area. There was less scar tissue in the 63 day animals, but the collagen had a more intense infrared signature. While we were able to image the wound area using infrared microspectroscopy, there was no statistical evidence for improvement in the wound healing process based on treatment type. In addition to the studies on OTC and quercetin, it was noticed that the inherently polarized light from a synchrotron source resulted in changes in the IR spectra. These changes were the result of the polarized synchrotron light interacting with the oriented collagen fibres in the wound healing area. Tissue sections from the Achilles tendon of a rat were used to model the effects of polarized light on the IR spectrum of oriented samples.
ISBN
9780494643013
0494643013