Item – Theses Canada

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OCLC number
756267026
Link(s) to full text
LAC copy
LAC copy
Author
Kena-Cohen, Veronique,1983-
Title
Defining a physiological model of classical activation in microglia.
Degree
M. Sc. -- University of Toronto, 2008
Publisher
Ottawa : Library and Archives Canada = Bibliothèque et Archives Canada, [2010]
Description
2 microfiches
Notes
Includes bibliographical references.
Abstract
<?Pub Inc> Microglia are resident immune cells of the central nervous system that can become activated following injury, disease, or infection. 'In vitro, ' they can be activated by different stimuli, which determine the inflammatory phenotype they will develop. In this thesis, stimulating microglia with tumor necrosis factor-[alpha] (TNF[alpha]) and interferon-[gamma] (IFN[gamma]) resulted in classical activation, characterized by proliferation, increased transcription of complement receptor 3 and major histocompatibility class II molecules, and elevated production and transcription of interleukin-1[beta] and nitric oxide. Stimulation with TNF[alpha] and IFN[gamma] also changed the intensity of phosphorylated (activated) cyclic adenosine monophosphate response element binding protein (pCREB) immunoreactivity in microglia. Specifically cells were differentiated into populations with either high or low pCREB intensity. This was the first example of such a response in microglia. Furthermore, these results were representative of what occurred 'in vivo,' after ICH. Thus, the characterization of this model will be useful for future studies of this and other intracellular pathways involved in the classical activation of microglia.
ISBN
9780494587966
0494587962