Item – Theses Canada

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OCLC number
731320130
Link(s) to full text
LAC copy
LAC copy
Author
Reiz, Bela.
Title
Microwave-assisted acidbase hydrolysis and mass spectrometry for prion structure characterization.
Degree
M. Sc. -- University of Alberta, 2009
Publisher
Ottawa : Library and Archives Canada = Bibliothèque et Archives Canada, [2010]
Description
2 microfiches
Notes
Includes bibliographical references.
Abstract
Proteinaceous infectious particles are endogenous proteins that by misfolding can cause a variety of fatal neurodegenerative diseases, which can affect both humans and animals. Characterization of the structural conformers, the dynamics and kinetics involved in the misfolding of this protein could be helpful in the design and development of diagnostic tools and therapeutic products. In this work, we examined the use of microwave-assisted acid/base hydrolysis coupled with mass spectrometry for the characterization of the rSHPrP90-231 prion construct. We have identified an acid hydrolysis resistant core suggesting that the conformational conversion and aggregation of the protein involves part of [alpha]-helix 2, most part of [alpha]-helix 3 and the loop between these two helices when referred to the native PrP monomer. Circular dichroism, dynamic light scattering and electrophoresis data has also confirmed conversion and aggregation or oligomerization of the prion protein.
ISBN
9780494550014
0494550015