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Seevaratnam, Rajini,1985-
The effects of coffee, caffeine and chlorogenic acid supplementation on functional, disease and molecular outcome measures in male and female G93A mice, an animal model of amyotrophic lateral sclerosis (ALS).
M. Sc. -- York University, 2009
Ottawa :Library and Archives Canada = Bibliothèque et Archives Canada,[2010]
2 microfiches
Includes bibliographical references.
Oxidative stress is implicated in several neurodegenerative diseases including amyotrophic lateral sclerosis (ALS). The consumption of coffee and its constituents increases antioxidant status and reduces oxidative stress. We investigated the effect of coffee (COFF), caffeine (CAFN) and chlorogenic acid (CHLA) supplementation on functional and disease outcomes (food intake, body weight, body condition, motor performance, ability to move, clinical score), as well as markers of oxidative stress (4-HNE; 3-NY), antioxidant enzyme protein content (CuZn-SOD; Mn-SOD; CAT; GPx1; GR), inflammation (TNF-[alpha]), stress response (Hsp70), apoptosis (Bax; Bcl-2; pro- and cleaved-caspase 3), and motor neurons (ChAT; SMI-32; SMI-36) in the brain of 108 d old male (M) and female (F) G93A mice. Starting at age 40 d, 30 F and 21 M mice were randomly divided into eight groups: control (CNTL: 8 F, 6 M), COFF (7 F, 5 M), CAFN (8 F, 5 M) and CHLA (7 F, 5 M). 'Among males', COFF, CAFN, and CHLA proved beneficial with COFF offering the most profound effects of improved clinical score, reduced 4-HNE and 3-NY, increased antioxidant enzyme protein content, reduced TNF-[alpha], decreased pro-apoptotic Bax and Bax/Bcl-2, and increased motor neuron protein content. ' Among males', despite the positive effect of reduced 4-HNE content, CAFN had several negative effects including down-regulated antioxidant enzyme protein content, up-regulated TNF-[alpha], decreased Bcl-2, increased pro-apoptotic protein content, and reduced motor neuron content. Extrapolating these results to human ALS patients, COFF, CAFN, and CHLA supplementation prove advantageous to men, whereas CAFN supplementation proves harmful to women.