Item – Theses Canada

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OCLC number
651116360
Link(s) to full text
LAC copy
LAC copy
Author
Huang, Chun Bei.
Title
Proteomic investigation of biosynthesis exopolysaccharide by Ophiostoma ulmi.
Degree
M.A. Sc. -- University of Toronto, 2007
Publisher
Ottawa : Library and Archives Canada = Bibliothèque et Archives Canada, [2009]
Description
2 microfiches
Notes
Includes bibliographical references.
Abstract
The dutch elm tree disease casual agent, 'Ophiostoma ulmi,' was investigated for its ability to produce exopolyssacharides (EPS), which have great industrial potential. This is the first proteomic work based on ' O. ulmi.' EPS biosynthesis was studied using proteomics tools with the purpose of identifying the proteins/enzymes that were responsible for EPS biosynthesis. The extracellular 'O. ulmi' protein profile under various culture conditions was obtained with SDS PAGE and then compared. The gel bands were then excised and treated with trypsin, followed by protein identification by a matrix assisted laser desorption/ionization time of flight (MALDI-TOF) instrument. It was found that sucrose synthase, acyl carriers proteins and various transferases were induced by substrate (sucrose), or nitrogen source (asparagine), which indicated an intracellular biosynthesis pathway. However, glucoamylase, another protein that could be involved in the extracelluar synthesis also appeared. A future study of intracellular proteins is suggested.
ISBN
9780494400586
0494400587