Item – Theses Canada

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OCLC number
64669353
Link(s) to full text
LAC copy
LAC copy
Author
Cui, Ke,1978-
Title
Effect of oxidative stress and nutraceutical antioxidants on the structural and functional integrity of the mitochondrial genome.
Degree
M. Sc. -- Université Laval, 2004
Publisher
Ottawa : Library and Archives Canada = Bibliothèque et Archives Canada, [2005]
Description
2 microfiches.
Notes
Includes bibliographical references.
Abstract
Reactive oxygen species (ROS) produced in normal physiological or pathological conditions can damage mitochondrial DNA (mtDNA) to different extents. In Part l, we have tried to concentrate on the following points dealing with the role of antioxidants in protecting mtDNA against oxidative stress: (1) the complex interactions and complementary interrelationships between oxidative stress, mitochondrial dysfunction and various forms of neural degeneration; (2) fractionation and isolation of substances with antioxidant properties from plant materials which are extensively used in the human diet, and therefore, can be expected to be less toxic in any pharmacological intervention; (3) developments of methodologies for the assay of oxidative stress and determination of biological activities of exogenous and endogenous antioxidants, and (4) presentation of simple procedures based on PCR and restriction fragment length polymorphism (RFLP) of the resulting amplicon for investigations of structural alterations in mtDNA. In Part 2, we have developed experimental approaches for the determination of relative mtDNA copy numbers in different mouse tissues so that the roles of mtDNA in cells with different levels of metabolic acivities can be more clearly understood. (Abstract shortened by UMI.)
ISBN
0612950344
9780612950344