Item – Theses Canada

​
OCLC number
64500215
Link(s) to full text
LAC copy
LAC copy
Author
Huang, Kuo-Cheng,1978-
Title
The effect of expression of the coxsackie and adenovirus receptor on glioma growth and invasion.
Degree
M. Sc. -- McGill University, 2004
Publisher
Ottawa : Library and Archives Canada = Bibliothèque et Archives Canada, [2005]
Description
2 microfiches.
Notes
Includes bibliographical references.
Abstract
The paucity of CAR in malignant glioma cell lines and grade IV primary astrocytomas (glioblastoma multiforme) suggest that CAR expression may be disadvantageous to malignant gliomas. The present study directly tested this hypothesis by determining the effect of forced CAR expression on the invasion and growth of the human glioma cell line U87-MG expressing no detectable levels of CAR. We also studied the requirement of the cytoplasmic domain of CAR in mediating for these effects. We have found that forced expression of the full-length forms of mCAR1 isoform in U87-MG cell lines (i) does not affect their growth 'in vitro' in monolayer, but does significantly reduce the tumor volumes generated by implanted glioma cells in mouse brains ' in vivo'; and (ii) reduced invasion by glioma cells in a three-dimensional spheroid model. In contrast, expression in U87 cells of the tailless-CAR having the entire cytoplasmic domain deleted except for the first two intracellular juxtamembrane cysteine-cysteine amino acids did not significantly reduce the invasion rate of the glioma spheroid 'in vitro', nor did it reduce the tumor volume generated 'in vivo' in the brain. Together these results suggest that the paucity of CAR expression in malignant gliomas may confer the tumors selective advantage in growth and invasion.
ISBN
0612986616
9780612986619