Item – Theses Canada

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OCLC number
56806443
Link(s) to full text
LAC copy
LAC copy
Author
Patton, Joseph John,1971-
Title
Identification and characterization of genes in hybrid poplar (Populus trichocarpa x P. deltoides) upregulated by mechanical wounding and herbivory by the forest tent caterpillar (Malacosoma disstria).
Degree
M. Sc. -- University of Alberta, 2003
Publisher
Ottawa : National Library of Canada = Bibliothèque nationale du Canada, [2004]
Description
2 microfiches.
Notes
Includes bibliographical references.
Abstract
In hybrid poplar ('Populus trichocarpa' * ' P. deltoides'), mechanical wounding, FTC herbivory, and methyl jasmonate are known to induce expression of polyphenol oxidase (PPO) and trypsin inhibitor (TI). To identify novel wound-responsive genes, a suppressive subtractive hybridization (SSH) library was constructed from young leaves showing strong systemic-induction of PPO and TI after wounding. Macro-arrays were differentially screened using 32P-radiolabelled cDNA representing total mRNA from mechanically wounded, FTC browsed, and control leaves. Wound-induced cDNAs were sequenced and their predicted peptide sequences were used to query databases to predict their function. Induction locally and systemically was confirmed in poplar leaves after mechanical wounding. Herbivory was compared to mechanical wounding and the chemical elicitors methyl jasmonate and salicylic acid. Unwounded poplar tissues were examined to explore developmental gene expression of the wound-induced genes. The results presented in this thesis have expanded the number of known wound-induced genes in the poplar system.
ISBN
061282327X
9780612823273