Item – Theses Canada

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OCLC number
495695100
Link(s) to full text
LAC copy
LAC copy
Author
Salleh, Ayesha,1980-
Title
Influences of neonatal environments on stress-induced behaviours, mammary gland morphology, and estrogen receptor alpha and p53 expression in Balbc mice.
Degree
M. Sc. -- Trent University, 2008
Publisher
Ottawa : Library and Archives Canada = Bibliothèque et Archives Canada, [2008]
Description
2 microfiches
Notes
Includes bibliographical references.
Abstract
Early-life experiences influence HPA axis development, affecting hormone levels, stress-induced behaviours and possibly normal and malignant mammary gland development. Influences of neonatal experiences on stress-induced behaviours and mammary gland development were examined in pubertal (PND 30-35) and adult (PND 55-65) mice. Similar to other studies, immobility in the forced swim test was higher in maternally separated (MS; 4 hrs/day; PND2-22) compared to handled (H; 15 mins/day; PND2-22) mice (p's<0.05), but this difference was age- and gender-dependent. Additionally, neonatal manipulations influenced mammary gland growth, whereby H compared to MS and typically reared (TR; control) adult mammary glands were significantly more differentiated (p's<0.05). Examination of mRNA and protein expression of p53 and estrogen receptor alpha (ER[alpha]), found that only ER[alpha] protein expression was higher in adult mammary glands of both H and MS, compared to TR mice (p's<0.05). These results suggest that neonatal manipulations influence mammary gland development, but the interaction is complex. 'Keywords': Neonatal manipulation, depressive behaviour, mammary gland development, estrogen receptor alpha, tumour suppressor gene p53.
ISBN
9780494317150
0494317159