Item – Theses Canada

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OCLC number
458722138
Link(s) to full text
LAC copy
LAC copy
Author
Sun, Yang.
Title
Establishing a superior tetracycline-inducible gene expression system for mouse CD200, a potential therapeutic immunosuppressive molecule.
Degree
M. Sc. -- University of Toronto, 2007
Publisher
Ottawa : Library and Archives Canada = Bibliothèque et Archives Canada, [2007]
Description
2 microfiches
Notes
Includes bibliographical references.
Abstract
CD200 (originally called OX2) is a membrane glycoprotein expressed by a variety of cell types, including lymphocytes, neurons and endothelium. It is the ligand for CD200 receptors (CD200Rs) whose expression is limited to hematopoietic cells, especially myeloid cells. A number of 'in vivo ' studies have implied that the interaction of CD200 with CD200R1 may inhibit a variety of immune responses, including graft rejection, autoimmune disease, fetal loss syndrome, and cancer. To study further the biological function of CD200 and the CD200Rs, our lab produced TRE-CD200-EGFP transgenic mice using a Tet-On inducible system. This thesis evaluated the inducible function of rtTA2s-M2, a new generation of Tet-On regulator, and compared it to the old version of rtTA 'in vitro' and 'in vivo.' The data suggest that induction of the CD200-EGFP transgene in rtTA2s-M2 transgenic mice is superior to that achieved in rtTA transgenic mice following doxycycline feeding 'in vivo.'
ISBN
9780494273739
0494273739