Item – Theses Canada

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OCLC number
456826816
Link(s) to full text
LAC copy
LAC copy
Author
Gordon, Roni David.
Title
Stuctural insights into rabbit N-acetylglucosaminyltransferase I donor specificity, acceptor binding and catalysis.
Degree
M. Sc. -- University of Toronto, 2007
Publisher
Ottawa : Library and Archives Canada = Bibliothèque et Archives Canada, [2007]
Description
2 microfiches
Notes
Includes bibliographical references.
Abstract
N-acetylglucosaminyltransferase I (GnT I) is a carbohydrate processing enzyme in the Golgi that initiates the conversion of high-mannose oligosaccharides to complex and hybrid structures in the biosynthesis of N-linked glycans in the secretory pathway. Building on the previously determined x-ray crystal structure of the nucleotide-bound complex of GnT I in the presence of its native substrate, we obtained a series of highresolution nucleotide-sugar analogue complexes that provide structural insight into nucleotide-sugar recognition and underscore the importance of the sugar C2 position in catalysis. In particular, our crystallographic studies of GnT I provide evidence for the importance of the GlcNAc moiety and its N-acetyl group in substrate binding, as well as the role played by a flexible loop in substrate binding and product release. Moreover, by modeling the oligosaccharide substrate in the active site, we were able to suggest a rationalization for the main determinants of GnT I oligosaccharide specificity.
ISBN
9780494272695
0494272694