Item – Theses Canada

​
OCLC number
430650890
Link(s) to full text
LAC copy
LAC copy
Author
Andrews, Bryan.
Title
Pinguid, the Drosophila homolog of Huntingtin interacting protein 14, encodes an essential protein involved in TGF beta signalling.
Degree
M. Sc. -- Simon Fraser University, 2005
Publisher
Ottawa : Library and Archives Canada = Bibliothèque et Archives Canada, [2007]
Description
1 microfiche
Notes
Includes bibliographical references.
Abstract
To elucidate the cellular function of Huntingtin interacting protein 14 (Hip14), a genetic approach using 'Drosophila' was initiated. The gene encoding Pinguid was identified as the closest ' Drosophila' homolog to human Hip14 with 44% identity and 59% similarity. A P-element insertion in the 5' UTR of 'pinguid' was used for carrying out a transposase mediated P-element excision screen. Subsequent genetic and molecular experiments confirmed that 'pinguid' encodes an essential gene. Five 'pinguid' alleles were identified: three excision alleles and two EMS-induced alleles. No visible defects were observed in the mutants since the morphology and patterning of the embryos appeared normal, CNS development appeared wild type, and pharate adults had no visible external defects. However, the ectopic expression of 'pinguid ' identified genetic interactions with components of the TGF[beta] signalling pathway. Strong genetic interactions were seen with 'short gastrulation, crossveinless 2, baboon,' and the 'ecdysone receptor '. 'Keywords.' Huntingtin interacting protein 14, palmitoylation, ' Drosophila', wing development, TGF[beta] signalling
ISBN
9780494240274
049424027X