Item – Theses Canada

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OCLC number
30811714
Author
Wang, Hui,1958 June 7-
Title
In vivo and in vitro study of murine cytokine gene expression via RNA and protein analysis.
Degree
M. Sc. -- University of Manitoba, 1991
Publisher
Ottawa : National Library of Canada = Bibliothèque nationale du Canada, 1993.
Description
2 microfiches.
Notes
University Microfilms order no. UMI00382593.
Includes bibliographical references.
Abstract
This study has focused on the regulation of expression of Cytokines (CK) genes induced by extracellular stimuli. The expression of three CKs IL-4, IFNr and IL-2, which play important roles in allergic immune responses, was investigated. Conclusions of the study include (i) in vivo administration of anti-CD3 mAb to naive mice induces a rapid, transient and synchronous expression of IL-4 and IFNr genes; (ii) the IL-4 and IFNr mRNAs are readily translated to biologically active proteins; (iii) IL-2 gene expression is not affected by anti-CD3 stimulation in vivo and no functional protein detectable in the bioassay with mRNA synthesis; (iv) differential regulation of IL-2 gene and IL-4, IFNr genes can be observed with in vivo stimulation of anti-CD3; (v) in vitro stimulation of naive spleen cells with anti-CD3 induces a late, transient and synchronous expression of IL-4 and IFNr genes, with their functional proteins translated; (vi) the study has provided evidence for mature IL-4 secreting T cell presence in vivo. (Abstract shortened by UMI.)
ISBN
0315770341
9780315770348