Item – Theses Canada

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OCLC number
234284898
Author
Fussner, Eden Margaret.
Title
Heterodimerization and oligomerization of the BTB domain.
Degree
M. Sc. -- University of Toronto, 2006
Publisher
Ottawa : Library and Archives Canada = Bibliothèque et Archives Canada, [2007]
Description
1 microfiche
Notes
Includes bibliographical references.
Abstract
The BTB (B_road complex, T_ramtrack and B_ric à Brac) domain is a versatile protein-protein interaction motif found in Eukaryotes and poxvirus. The human genome encodes 183 BTB-containing proteins, which function in an array of biological processes including protein degradation, cytoskeleton regulation, ion transport, and transcription regulation. Here we systematically investigate whether the BTB domains from a subset of BTB zinc finger transcription factors form homo- or heterotypic BTB-BTB associations using a size exclusion chromatography assay. Additionally, we used an 'in vitro' FRET-based assay to evaluate the oligomerization capacity of the BTB domain from the transcription factor Kaiso. We found that the BTB domains from these transcription factors facilitate both homomeric and heteromeric associations, and may be able to oligomerize in the presence of a DNA template.
ISBN
9780494160626
0494160624