Item – Theses Canada

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OCLC number
1335044191
Link(s) to full text
LAC copy
Author
Phan, Van Hong.
Title
Evaluation of GCaMP3 Protoplasts for the Analysis of Ca2+ Signaling in Plant Immunity.
Degree
M.Sc. -- University of Toronto,, 2018.
Publisher
[Toronto, Ontario] : University of Toronto, 2018
Description
1 online resource
Abstract
Ca2+ serves as a common second messenger in diverse eukaryotic cells. However, measuring in planta Ca2+ levels has been difficult. Recently, the fluorescence-based Ca2+ visualization indicator, GCaMP3 has emerged as a tool to visualize Ca2+ signals in plant cells; in particular, stress-related Ca2+ signals were successfully captured in GCaMP3 expressing leaf discs. In this study, the usage of GCaMP3 in protoplasts was evaluated. Upon stress-related stimuli, stress-induced Ca2+ signals were captured in Arabidopsis thaliana protoplasts using a fluorescence microscope and a plate reader. The signal pattern was faster and quicker but fundamentally similar to that of leaf discs, indicating that protoplasts can be used for Ca2+ visualization study. Furthermore, transfection of GCaMP3 in protoplasts was evaluated. Upon the bacterial elicitor flg22 treatment, Ca2+ signal was seen once by transfecting GCaMP3. Taken together, protoplasts can be used for investigating stress-induced Ca2+ signals using GCaMP3; however, transfection protocol has to be optimized.
Other link(s)
tspace.library.utoronto.ca
tspace.library.utoronto.ca
hdl.handle.net
Subject
Calcium
Calcium signaling
Fluorescence
Plant immunity
Protoplasts