Item – Theses Canada

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OCLC number
1334506813
Link(s) to full text
LAC copy
Author
Curak, Jasna.
Title
Investigating the ERBB Protein Interactome.
Degree
MAST -- University of Toronto, 2011.
Publisher
[Toronto, Ontario] : University of Toronto, 2011
Description
1 online resource
Abstract
The erythroblastoma (ErbB) receptor family consists of four members that are implicated in many human cancers. To gain insight into their biological function, we investigated their interacting partners to derive a comprehensive protein interaction network. Using the membrane yeast two-hybrid (MYTH) system we probed the ErbB2, ErbB3, and ErbB4 interaction space and validated a subset of interacting partners using the luminescence-based mammalian interactome mapping (LUMIER) system. The integrated use of these two complementary protein interaction technologies generated high confidence data and identified many novel ErbB binding partners, a subset of which was supported by co-immunoprecipitation in the breast adenocarcinoma cell line Sk-Br-3 including the GPCR GPRC5B, the cysteine protease CAPN1, and WIF1, a secreted protein containing 5 EGF domains that may represent a novel ErbB ligand. Our systematic approach offers an unbiased systems level view that may identify novel drug targets and contribute to therapeutic research.
Other link(s)
tspace.library.utoronto.ca
hdl.handle.net
Subject
ErbB receptors
LUMIER