Item – Theses Canada

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OCLC number
1032954084
Link(s) to full text
LAC copy
LAC copy
Author
Odedra, Devangbhai.
Title
Endothelial Cells Guided by Immobilized Gradients of Vascular Endothelial Growth Factor on Porous Collagen Scaffolds.
Degree
MAST -- University of Toronto, 2011
Publisher
Toronto : University of Toronto, 2011.
Description
1 online resource
Notes
Includes bibliographical references.
Abstract
A key challenge in tissue engineering is overcoming cell death in the scaffold interior due to the limited diffusion of oxygen and nutrients therein. We hypothesized here that immobilizing a gradient of vascular endothelial growth factor (VEGF-165) would guide endothelial cells into the interior of the scaffold thereby enhancing angiogenesis. The protein was immobilized onto a collagen scaffold through carbodiimide chemistry by one of the three methods experimented: placing 5 µl of the solution at the center of the scaffold to create a ~2 ng/ml/mm gradient in a radial direction. D4T endothelial cells were observed to be guided by this VEGF-165 gradient deep into the center of the scaffold compared to both uniformly immobilized VEGF-165 and VEGF-free controls. We concluded that the VEGF-165 gradient scaffolds promoted the migration, and not proliferation, of cells deep into the scaffold. These gradient scaffolds provide the foundation for future in vivo tissue engineering studies.
Other link(s)
hdl.handle.net
tspace.library.utoronto.ca
Subject
gradient.
VEGF.
Tissue Engineering.
collagen.
immobilization.
0541.