Item – Theses Canada

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OCLC number
1032910936
Link(s) to full text
LAC copy
LAC copy
Author
Rogalska, Tetyana.
Title
Requirement(s) for the Replication of Lucerne Transient Streak Virus Satellite RNA.
Degree
MAST -- University of Toronto, 2012
Publisher
Toronto : University of Toronto, 2012.
Description
1 online resource
Notes
Includes bibliographical references.
Abstract
The satellite RNA of Lucerne Transient Streak Virus (LTSV) is a 322-nucleotide, single-stranded circular RNA that has a rod-like structure very similar to that of viroids. As it does not encode any translation products and cannot replicate independently of a helper virus, the satellite RNA is proposed to rely on viral-encoded proteins for the replication and/or cell-to-cell movement that facilitate its systemic infection in a host. To investigate the requirements for replication of the LTSV satellite RNA, transgenic plant systems were generated to express the viral RNA-dependent RNA polymerase and predicted viral transport protein independently as well as in combination. Results of infectivity assays of these transgenic lines demonstrated for the first time that the viral-encoded RNA-dependent RNA polymerase is necessary and sufficient for the replication of LTSV satellite RNA, and that no additional viral proteins are required for its cell-to-cell or systemic transport.
Other link(s)
hdl.handle.net
tspace.library.utoronto.ca
Subject
Lucerne Transient Streak Virus.
Satellite RNA.
Viral RNA-dependent RNA polymerase.
Viroid.
0720.
0307.
0410.