Item – Theses Canada

​
OCLC number
1019473264
Link(s) to full text
LAC copy
Author
Archambault-Wallenburg, Marika.
Title
Two-photon Microscopy and Polarimetry for Assessment of Myocardial Tissue Organization.
Degree
M. Sc. -- University of Toronto, 2010
Publisher
Ottawa : Library and Archives Canada = Bibliothèque et Archives Canada, 2011.
Description
1 online resource
Notes
Includes bibliographical references.
Abstract
<?Pub Inc> Optical methods can provide useful tissue characterization tools. For this project, two-photon microscopy and polarized light examinations (polarimetry) were used to assess the organizational state of myocardium in healthy, infarcted, and stem-cell regenerated states. Two-photon microscopy visualizes collagen through second-harmonic generation and myocytes through two-photon excitation autofluorescence, providing information on the composition and structure/organization of the tissue. Polarimetry measurements yield a value of linear retardance that can serve as an indicator of tissue anisotropy, and with a dual-projection method, information about the anisotropy axis orientation can also be extracted. Two-photon microscopy results reveal that stem-cell treated tissue retains more myocytes and structure than infarcted myocardium, while polarimetry findings suggest that the injury caused by temporary ligation of a coronary artery is less severe and more diffuse that than caused by a permanent ligation. Both these methods show potential for tissue characterization.
ISBN
9780494724972
0494724978