Item – Theses Canada

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OCLC number
1019464625
Link(s) to full text
LAC copy
Author
Wilson, Elizabeth.
Title
Synthesis of a Novel Acyl Phosphate Cross-linker and its Modification of Hemoglobin.
Degree
M. Sc. -- University of Toronto, 2012
Publisher
Ottawa : Library and Archives Canada = Bibliothèque et Archives Canada, 2013.
Description
1 online resource
Notes
Includes bibliographical references.
Abstract
<?Pub Inc> Hemoglobin-based oxygen carriers (HBOCs) are of great interest for their potential as a safer alternative to blood transfusions. To overcome the vasoactivity associated with small HBOCs, our group is interested in connecting two hemoglobin tetramers together, forming "bis-tetramers". Bis-tetramers have previously been synthesized by our group, but yield and purity of the resulting solutions have been low and hindered their usefulness for trials. A new cross-linker was designed in an attempt to improve yield. This thesis describes the synthesis of an acyl phosphate cross-linker 'N'-[bis(sodium methyl phosphate)isophthalyl]-4-azidomethylbenzoate (5), its modification of hemoglobin and subsequent purification attempts of the resulting solution. Cross-linker 5 was found to be selective to [beta]-[beta]-crosslinking and produced singly modified subunits as byproducts. Attempts to purify the resulting reaction mixture by heating resulted in the decomposition of the azide group on the cross-linker, which was critical for the coupling step. Efforts to overcome this problem were unsuccessful.
ISBN
9780494929698
0494929693
9780494929698