Item – Theses Canada

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OCLC number
1017554351
Link(s) to full text
LAC copy
Author
Lipsett, Mark Andrew,1974-
Title
Pancreatic plasticity during chronic glucose infusion.
Degree
M. Sc. -- Simon Fraser University, 2000
Publisher
Ottawa : National Library of Canada = Bibliothèque nationale du Canada, [2001]
Description
2 microfiches
Notes
Includes bibliographical references.
Abstract
Diabetes mellitus occurs through a deficit in the pancreatic [beta]-cell mass. Understanding the mechanism(s) of [beta]-cell mass growth could enable new treatment regimens allowing for greater control of blood glycemia. Male Sprague-Dawley rats (200-250g) were infused with either saline (0.9%) or 50% glucose solution at 2 ml/hour for 0, 1, 2, 3, 4, 5 or 6 days. The increase in [beta]-cell mass was associated with an increase in [beta]-cell replication and average cell size, though the increases in these parameters were not sufficient to account for the total [beta]-cell mass growth. Pancreatic sections were analysed for focal area tissue, duct cell replication and single [beta]-cell number to quantify the contribution of [beta]-cell neogenesis. The findings demonstrate that [beta]-cell neogenesis is an important component of [beta]-cell mass expansion during chronic glucose infusion in rats. Acinar cell transdifferentiation into [beta]-cells and dedifferentiation into neogenic focal areas suggest that acinar cells possess the ability to increase [beta]-cell mass when stimulated appropriately. (Abstract shortened by UMI.)
ISBN
0612513963
9780612513969