Item – Theses Canada

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MacIntyre, Terence M.(Terence Michael),1972-
Acetyl CoA carboxylase, adipocyte P2, lipoprotein lipase, and hormone-sensitive lipase mRNA levels in the ovine adipose tissues and their relationship with carcass fat.
M. Sc. -- Dalhousie University, 1999
Ottawa : National Library of Canada = Bibliothèque nationale du Canada, [2001]
2 microfiches
Includes bibliographical references.
Recent advancements in molecular biology have provided an opportunity to identify genetic markers that could facilitate identification of lean animals at an early age. The level of RNA of genes controlling fat metabolism could be a useful source of information in choosing potential candidate genes. The objective of this study was to obtain preliminary data on acetyl co-A carboxylase (ACC), adipocyte P2 (aP2), hormone sensitive lipase (HSL), and lipoprotein lipase (LPL) as potential markers for carcass composition. Two experiments were performed. Karakul (n = 5), Rideau Arcott (n = 5), Dorset (n = 5), Texel (n = 3) and Texel-Dorset cross (n = 5) animals were used in the first experiment, and represented substantial differences in carcass composition. The mRNA levels of the four genes were measured in the subcutaneous fat samples collected at slaughter. In the second experiment, fat biopsies were taken from the tail of five Karakul lambs at monthly intervals from one to seven months of age to evaluate gene expression during growth. Growth of the fat depot was estimated by measuring length, thickness and circumference of the tail at the time of taking fat biopsies. (Abstract shortened by UMI.)