Item – Theses Canada

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Morrison, Andrew John.
Heat shock transcription factors and the hsp70 induction response in neural and non-neural tissues of the hyperthermic rat during postnatal development.
M. Sc. -- University of Toronto, 2000
Ottawa :National Library of Canada = Bibliothèque nationale du Canada,[2001]
2 microfiches
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Heat shock transcription factor 1 (HSF1) levels increase in brain regions during postnatal rat development, but decrease in kidney and heart, HSF1 protein levels in brain and kidney correlate with levels of HSF DNA-binding activity and the magnitude of hsp70 protein induction after thermal stress in both neonatal and adult rats. There appears to be more HSF1 protein in adult brain than is needed for induction of hsp70 after thermal stress, suggesting that HSF1 may have other roles in addition to its role as a stress-inducible activator of heat shock genes. HSF2 protein levels decline during postnatal rat development in brain regions, kidney and heart. Gel mobility shift analysis showed that HSF2 is not in a DNA-binding form in the neonatal rat, suggesting that HSF2 may not be involved in the constitutive expression of heat shock proteins in early postnatal development. There is no apparent correlation between levels of HSF2 protein and basal levels of hsp90, hsp70, hsc70 and hsp60.